Construction of Yeast One-Hybrid Library of Alternaria oxytropis and Screening of Transcription Factors Regulating swnK Gene Expression

The indolizidine alkaloid-swainsonine (SW) is the main toxic component of locoweeds and cause locoweed poisoning in grazing animals. endophytic fungi, Alternaria Section Undifilum spp., are responsible for biosynthesis SW locoweeds. swnK gene a multifunctional complex enzyme encoding fungal biosynthesis, its product plays key role multistep catalytic synthesis by fungi using pipecolic acid as precursor. However, transcriptional regulation mechanism still unclear. To identify regulators involved locoweeds, we first analyzed upstream non-coding region A. oxytropis UA003 strain predicted high activity combined with dual-luciferase reporter assay. Then, yeast one-hybrid library was constructed, regulatory factors that may bind to high-transcriptional were screened this system. results showed located at −656 bp −392 gene. A total nine candidate regulator molecules, including C2H2 type transcription factor, seven annotated proteins, an unannotated protein, out through Y1H system, which bound This study provides new insight into lays foundation further exploration mechanisms